Referring the blaze that claimed the lives of 70 people in June, the rapper said: 'Yo Theresa May, where's the money for Grenfell?
She said making the symbolic statement to "millions and millions of people, not just in the United Kingdom but all over the world, it does make a difference, it does make a change because we are standing, we are protesting in our own way and this is how we're going to make a difference".
The Brit Awards weren't the first time Stormzy has called out Theresa May and the Conservative Government.
The show also saw Oasis frontman Liam Gallagher perform Live Forever in a tribute to the victims of a suicide bombing attack on a venue hosting a concert by pop star Ariana Grande in Manchester in May a year ago, which killed 22 people.
She added: "Here's to more women on these stages, more women winning awards and more women taking over the world".
The rapper from Croydon, South London - whose real name is Michael Omari - won British Album of the Year, the top award of the night.
Yo Theresa May, where's that money for Grenfell?
"We are protesting in our own way and this is how we're going to make a difference".
"The public inquiry was set up by the Prime Minister to establish what went so tragically wrong and why people weren't listened to for so many years".
But the entire ceremony had a political edge, with many stars wearing white roses in solidarity with the Time's Up and #Metoo movements highlighting sexual harassment in the entertainment industry.
Lipa, 22, was able to beat the likes of Kate Tempest and Laura Marling for the British Female Solo Artist category. Albarn began a slurred speech before he was cut off: "This country is believe it or not quite a small little thing right, but it's a lovely place and part of a handsome world..."
He appeared on a charity single for the victims of the Grenfell disaster past year, and also called for justice for them at the Glastonbury festival, saying: "We urge the authorities to tell the fucking truth, first and foremost".
Ed Sheeran was presented with the Global Success award by Rolling Stones rocker Ronnie Wood, who had some high praise for the singer-songwriter.
Sheeran, who, despite releasing the biggest-selling album in the United Kingdom past year with "Divide", missed out on wins in the top categories.
Dua Lipa, 22, was one of the first to arrive and took the initiative a step further by actually carrying a rose. Paloma, who was nominated for Best British Female Solo Artist, said it was important pop stars used their fame to speak up for women.